356 Investigating for Extracellular Vesicles miRNA involved in the dialog between dermis and epidermis, as potential targets for antiaging interventions in skin

The aim of this work is to explore the communication between fibroblasts (dermis) and keratinocytes (epidermis) in process skin aging. We thought that Extracellular vesicles (EVs) secreted by aged or senescent might promote sustain epidermis In order get insight into a potential function EVs aging, we first investigated impact secretome on clonogenic keratinocytes. found containing secretomes young are able improve from old donors terms number Holoclones, Meroclones Paraclones. To further investigate role fibroblast EV process, purified analyzed content human proliferative dermal 6 catalogued therein contained miRNAs. interestingly 54 specific miRNAs donors. Among top 10 most abundant ones, were involved physiological aging photo-aging. Some them also cells, suggesting interplay cells promoting through keratinocyte stem cells. addition, identified 3 novel specifically expressed as new target prevent better understand rejuvenation effect skin, miRNA content. induce expression 126 miRNA. them, 70 usually re-expressed treated P. Bensadoun M. Gabanou participated equally work.